Senescent phenotypes and telomere lengths of peripheral blood T-cells mobilized by acute exercise in humans

Autor/innen

  • Richard J. Simpson Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA Autor/in
  • Cormac Cosgrove Biomedicine and Sports Science Research Group, School of Life Sciences, Edinburgh Napier University, 10 Colinton Road, Edinburgh EH10 5DT, Scotland UK Autor/in
  • Meng M. Chee Division of Cancer Sciences and Molecular Pathology, Department of Surgery, University of Glasgow, Queen Elizabeth Building, Glasgow Royal Infirmary, 10 Alexandra Parade, Glasgow G31 2ER, Scotland UK Autor/in
  • Brian K. McFarlin Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA Autor/in
  • David B. Bartlett Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA Autor/in
  • Guillaume Spielmann Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA Autor/in
  • Daniel P. O'Connor Laboratory of Integrated Physiology, Department of Health and Human Performance, University of Houston, 3855 Holman Street, Houston, Texas 77204, USA Autor/in
  • Hanspeter Pircher Institute for Medical Microbiology and Hygiene, Department of Immunology, University of Freiburg, Freiburg, Germany Autor/in
  • Paul G. Shiels Division of Cancer Sciences and Molecular Pathology, Department of Surgery, University of Glasgow, Queen Elizabeth Building, Glasgow Royal Infirmary, 10 Alexandra Parade, Glasgow G31 2ER, Scotland UK Autor/in

DOI:

https://doi.org/10.22029/eir.2010.1650

Schlagwörter:

CYTOMEGALOVIRUS-INFECTION, REPLICATIVE SENESCENCE, LYMPHOCYTE APOPTOSIS, PROLONGED EXERCISE, RECEPTOR KLRG1, E-CADHERIN, EXPRESSION, IMMUNOSENESCENCE, EFFECTOR, REDISTRIBUTION

Abstract

"Acute bouts of aerobic exercise are known to mobilize antigen-experienced CD8+ T-cells expressing the cell surface marker of senescence, KLRG1, into the blood. It is not known; however, if this is due to a selective mobilization of terminally differentiated T-cells (i.e. KLRG1+/CD28-/CD57+) or a population of effector memory T-cells (i.e. KLRG1+/CD28+/CD57-) that have not reached terminal differentiation. The aim of this study was to farther characterize KLRG1+ T-cells mobilized by acute exercise by assessing the co-expression of KLRG1 with CD28 or CD57 and to determine telomere lengths in the CD4+ and CD8+ T-cell subsets. Nine moderately trained male subjects completed an exhaustive treadmill running protocol at 80%. Blood lymphocytes isolated before, immediately after and 1 h after exercise were labelled with antibodies against KLRG1, CD28 or CD57, CD4 or CD8 and CD3 for 4-color,flow cytometry analysis. Telomere lengths in CD3+, CD4+ and CD8+ T-cells were determined using Q-PCR. The relative proportion of KLRG1+ cells among the CD8+ Teens increased by 40% immediately after exercise, returning to baseline I h later This was due to a mobilization of KLRG1+/CD28- (61% increase), KLRG1+/CD57+ (56% increase) and to a lesser extent, KLRG1+/CD57- cells (24% increase). Telomeres in CD8+ T-cells displayed an increased relative length immediately after exercise, whereas no change occurred for CD4+ or the overall CD3+ T-cells. In conclusion, the increased frequency of KLRG1+/CD8+ T-cells in blood after acute exercise is predominantly due to a selective mobilization of terminally differentiated T-cells. The increased relative telomere length in CD8+ T-cells after exercise might indicate that KLRG1+ cells mobilized by exercise are under stress or aberrant signaling-induced senescence (STASIS). We postulate that a frequent mobilization of these cells by acute exercise might eventually allow naive T-cells to occupy the ""vacant"" immune space and increase the naive T-cell repertoire."

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Veröffentlicht

2010-12-31

Ausgabe

Bd. 16 (2010)

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